Device
ElChu

Part:BBa_M36969:Design

Designed by: Dan Nelsen, Shivani Torres, Aaron Goebel   Group: Stanford BIOE44 - S11   (2012-12-06)

PoPS->SMTa Metallothionein(should be BBa_M36968


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1134


Design Notes

We made some modifications to the genetic code to delete repeated sequences. We also manually inputed a termination sequence prior to the Transcription Terminator as a secondary terminator.


The SMTa gene should be replaced with BBa_M36968, but the current limitations of the site prevent this

Source

THe SMTa gene comes from e.Coli

References